In vitro biological evaluation platform
ServicesHit Compound Discovery
Method Development
High-Throughput Screening
Hit Compound Discovery
Lead Optimization
Method Optimization
In Vitro Target Binding Experiments
In Vitro Functional Activity Experiments
Disease Model Establishment
Mechanism of Action Research
Cell Experiments mainly to Verify Targets
Method Research, Development, and Optimization
Enzyme and Cell Screening
Further Functional Verification Based on Cell Biology, Immunology, Signal Pathways, etc., FACS, qPCR, AlphaLISA, ELISA, Proliferation Experiments, etc.
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Main Target Types
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?Target Types |
?Signal Pathway |
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?GPCR |
?GTPγS (Non-Radiometric GTPγS Assays) ?cAMP (HTRF; agonist/antagonist) ?Calcium flux (Facs; IP-one) ?β-arrestin (Tango; PathHunter) ?Cellsensor (NFAT-bla; CRE-bla) |
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?Enzyme |
?Binding (Filtration; lantha, TDF) ?ADP quantitation (ADP-Glo, etc.) ?Phospho-substrate detection (HTRF, IMAP, Lantha screening, EZ reader, LC/MS/MS) ?Cellular phospho-protein detection (AlphaScreen, MSD, HCS, WB) |
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?Immuno-Oncology |
?Checkpoint blockade both biochemical Binding and cellular reporter assays ?In-house co-culture reporter assays ?Whole blood, PBMC, T cell, DC, B and NK cellular assays available ?Ex vivo assays MLR, co-culture, etc ?Cytokine release assays FACS, ELISA |
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?Ion channel |
?Binding (FP based binding) ?Electrophysiological functional assay (manual patch-clamp, automated patch-clamp) ?Fluorescence functional assay (FACS)? |
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?Nuclear Receptor |
?Reporter gene-based assay (transient transfection, stable cell line) ?FP based binding assay TaqMan based gene expression |
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?Cell line engineering |
?Stable overexpression (Flip-In, lentiviral and Retroviral system, Neon electroporation, T-REx system, HiBiT and PPI) ?Reporter, mutant, tag and tag-free expression ?Knock-out (KO) and Knock-In (KI) by CRISPR-Cas 9 ?Knock-down (KD) study by siRNA, shRNA and CRIPSR-Cas9 |
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Experimental Methods
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?Experiments |
?Methods |
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?Cellular Experiments |
?Cell Proliferation (2D, 3D, long-term (HTP), short-term conditions) |
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?Cell Viability Test (reagents such as CTG, CyQunat, CCK-8, MTT) |
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?Cell Migration and Invasion |
?Nuclear Transport? ?Experiments |
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?Apoptosis Experiment |
?Cell Cycle |
?Cell Colony Experiments |
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?Angiogenesis |
?Cell Line Engineering |
?High-Content Platform |
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?WB |
?qPCR |
?ELISA |
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?Immuno-oncology? ?Related Experiments |
?Immune cell isolation and culture, such as PBMC, T, B, NK, DC, and other cells |
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?Antibody Functional Evaluation，，， ，，，，，ADCC,ADCP，，， ，，，，，CDC，，， ，，，，， Co-Culture, MLR assays |
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?Innate and Acquired Immune Related Pathway Activity Test |
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?Immune cell activation, proliferation, differentiation |
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?Cytokine Detection |
?Flow Cytometry Analysis |
?Immune cell typing |
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?Immune checkpoint activity test |
?Tumor Killing Experiment |
?Tumor Infiltration? ?Experiment |
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?Enzyme Experiments? |
?Kniase assays |
?GPCR related methods |
?Protein interaction |
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?Methods: HTRF, ADP-Glo, Caliper, ELISA, Alphascreen, etc |
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?Other enzyme targets, such as phosphatase, protease, ubiquitin ligase, metabolic enzyme? ?classes |
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?Enzyme method development and optimization |
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?Phenotypic? ?and DNA Damage? |
?Protein acetylation |
?Protein methylation |
?mRNA modification |
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?DNA damage model |
?DNA exonuclease |
?RNA enzyme |
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?Reverse transcriptase? |
?Protein enzyme |
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